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. 2016 Sep 20;7:138. doi: 10.1186/s13287-016-0400-6

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Subpopulations of PBMCs and spleen cells in BALB/c mice immunized by MSCs and De-MSCs. BALB/c mice were injected with MMC-treated MSCs, Ob-MSCs, De-MSCs or Re-MSCs subcutaneously. PBMCs (5 d after injection) and splenocytes (7 d after injection) were collected and analyzed by FCM. CD4⁺CD25⁺ cells, CD80⁺CD11b⁺ cells, CD80⁺CD11c⁺ cells, and CD80⁺CD45R⁺ cells were gated as activated T cells, activated monocytes, activated dendritic cells and activated B cells respectively. (a–b) Subpopulations of PBMCs. (c–d) Subpopulations of splenocytes. Values of gated cells presented in the histogram were mean ± SD of three independent experiments. De-MSCs dedifferentiated mesenchymal stem cells, MSCs mesenchymal stem cells, Ob-MSCs MSC-derived osteoblasts, Re-MSCs osteoblasts derived from De-MSCs