Fig. 2.

Tyrosine kinase receptors and hormonal receptors expression in MBCDF’s subpopulations. Expression of all receptors was analyzed by Western blot. B2 and D5 were included in their opposite group as controls. Breast cancer cell line SKBR3 was used as control of HER2 expression, and MCF-7 cells were included as a positive control for the expression of ER and PR. a HER1, HER2, HER3, HER4, PDGFR, c-Kit, c-Met, IGF1-R expression. Actin was used as loading control. b VEGFR2 expression. Protein extracts from HUVECs were used as positive control and Tubulin as loading control. c ER and PR receptors expression. Actin was used as loading control