Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Sep 20;14:61. doi: 10.1186/s12958-016-0191-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 3 — Expressions of VEGFA and VEGFR2 mRNAs and proteins in GDM (n = 20) and control (n = 20) placental tissues by real-time PCR and Western immunoblotting. Relative quantification of VEGFA (a) and VEGFR2 (b) mRNA expression normalized to the expression of ACTIN in all the samples. Data were analyzed according to the 2^–ΔΔCT method. A representative immunoblots for VEGFA (c) and VEGFR2 (d) were shown. Immumoblots representing β-tubulin protein (middle panels) showed the loading equally total protein (25 μg). Semi-quantitative analyses of VEGFA and VEGFR2 immunoreactive proteins were performed relative to β-tubulin (bottom panels). The Y-axis represents the expression levels of VEGFA and VEGFR2 related to actin or tubulin. *P < 0.05, **P < 0.01, Student’s t test