Table 3.

Primer sequences for the 3′-UTR of SIRT1

Primer	Sequence	Restriction site
SIRT1-3′UTR for	5′-CTCGAGCTGTGAAACAGGAAGTAACAGACA-3′	XhoI
SIRT1-3′UTR rev	5′-GTTTAAA CTGGCAGTAATGGTCCTAGCTG-3′	PmeI

Restriction enzymes and the cutting sides of these enzymes are italicized