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. 2016 Sep 20;16:178. doi: 10.1186/s12883-016-0700-6

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Irradiation obviously promoted autophagy and obviously enhanced autophagy associated proteins expression in U87 GICs, and chloroquine can significantly inhibit the autophagy process which induced by radiation. a Representative images(×200) of the indicated regions of U87 GICs, which analyzed for LC3 expression by immunofluorescence analysis. b Quantitative analysis of the number of cells with punctuate GFP-LC3. c Authophagy-associated protein LC3 level in U87 GICs were examined by Western blotting. d Quantification of LC3 protein expression in U87 GICs treated by indicated treatments after normalization with GAPDH levels. Mean ± SD. n = 3. (*, p < 0.05;**p < 0.01)