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. 2016 Sep 20;16:178. doi: 10.1186/s12883-016-0700-6

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Combination treatment of irradiation and chloroquine have synergistically effects on decreasing the GICs’ tumorsphere numbers and diameters. a-d U87 GICs were treated as described before, then seeded in serum-free medium to generate tumorspheres,5 days after treatment, we counted and photographed total tumorspheres (a control group, b chloroquine group, c radiation group, d conbination group). e Quantitative analysis of the relative tumorsphere numbers treated by indicated treatments. f Quantitative analysis of the diameter of the tumorspheres treated by indicated treatments. (*, p < 0.05, **p < 0.01)