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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Nat Genet. 2016 Feb 15;48(4):367–373. doi: 10.1038/ng.3508

Figure 3. Effects of NUDT15 on thiopurine metabolism and cytotoxicity.

Figure 3

NUDT15 knockdown (NUDT15 KD, red) cells were established by lentiviral transduction of NUDT15-specific shRNA, and control cells (black) were transduced with non-targeted vectors. Thiopurine metabolites (cytosolic TGMP and TGTP, and DNA-incorporated thioguanine, [DNA-TG]) were analyzed after treatment with 1.25 μM of MP (A and B) or TG (E and F) for 48 hours. DNA-TG was measured in cells exposed to increasing concentrations of MP (D) and TG (H). Cytotoxicity was determined by MTT assay following 72-hour incubation with MP (C, 1.25 μM) or TG (G, 1.25 μM). Mean values are plotted in each panel with error bars indicating standard deviation from triplicates.