Fig. 6.

Dominant chimeric repressor-mediated suppression of ONAC095 function accelerated cold-induced ROS accumulation in ONAC095-SRDX plants. Three-week-old ONAC095-SRDX and WT plants were subjected to cold stress treatment by moving into a growth chamber with temperature of 4 °C for 1 day and leaf samples from cold stressed and unstressed plants were collected for different analyses. a and b In situ detection of H₂O₂ and superoxide anion in leaves by DAB and NBT staining, respectively. c and d Quantification of H₂O₂ level in leaves of ONAC095-SRDX and WT plants with or without cold (c) or drought (d) stress treatment. e and f Activity of SOD and CAT in leaves of ONAC095-SRDX and WT plants with or without cold stress treatment. g Expression of selected OsRboh genes in ONAC095-SRDX and WT plants with or without cold stress treatment. Relative expression levels were normalized by the transcript level of the Actin gene as an internal control and the expression level of the tested genes in WT plants under normal condition was set as 1. Data presented in (c–g) are the means ± SD from three independent experiments and columns with an asterisk indicate significant differences at p < 0.05 level between WT and OE/SRDX lines. WT, wild type; OE6, ONAC095-OE6; OE12, ONAC095-OE12; S2, ONAC095-SRDX2; S3, ONAC095-SRDX3