Figure 2.

Analysis of the mock community profiles. (a) Abundance profiles of the mock community as represented by PhyloTags (pooled from all five replicates), PacBio shotgun sequences and V4 iTags. Nocardiopsis dassonvillei, which was added at very low relative abundance, was exclusively detected in the PacBio shotgun data set. Additional contaminant OTUs were found only in the V4 iTags (Supplementary Table 3). (b) Spearman's rank correlation coefficients and corresponding P-values were calculated to evaluate the strength of relationships between various sequence data sets. (c) Principal coordinate analysis (PCoA) of microbial community structures at various Sakinaw Lake depths according to PhyloTags and iTags. Mean PCoA distances between iTag and PhyloTag pairs of the same depth are stated within parentheses in the legend. The inset shows depths 50 –120 m reanalyzed for higher resolution.