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. 2016 Sep 18;22:3301–3308. doi: 10.12659/MSM.900487

Figure 2.

Figure 2

Inhibition of miR-17 downregulates hPASMC proliferation. hPASMCs were transfected with anti-miR-17 or control miRNAs. (A) At 48 h after transfection, miR-17 level was determined by using qRT-PCR. (B) Proliferation of hPASMCs transfected with anti-miR-17 was determined by using CellTiter-Glo Luminescent cell viability assay kit. (C, D) Western blot analysis was conducted to detect the protein expression level of the proliferation marker PCNA in hPASMCs transfected with anti-miR-17. Data are expressed as the mean ±SD (n=3). ** P<0.01, *** P<0.001.