Figure 5.

MFN2 is essential for the miR-17-mediated regulation of proliferation and apoptosis in hPASMCs. hPASMCs were co-transfected with anti-miR-17 or anti-miR-NC and with either siMFN2 or siNC, and then incubated for 48 h. (A) Proliferation assay was performed with CellTiter-Glo reagent. (B) The apoptosis rate was determined by FCM with Annexin V/PI staining. (C) The activity of Caspase-3 was measured using a Caspase-3 colorimetric assay kit. (D) To determine the protein expression of pro-apoptotic protein cleaved Caspase-3 and PCNA, cells were harvested 48 h post-transfection and then subjected to Western blot analyses. Data are expressed as the mean ±SD (n=3). * P<0.05, ** P<0.01, *** P<0.001.