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. 2015 Dec 11;10(6):1383–1399. doi: 10.1038/ismej.2015.214

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Copyright © 2015 International Society for Microbial Ecology

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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Metagenomic fragment recruitment results (a) showing a high degree of coverage when queried against Ca. Nitromaritima SAGs (in bold red) from the Red Sea (SCGC AAA799-C22) and the Pacific Ocean (SCGC AA288-L16) relative to the genomes of N. gracilis (in blue) and other NOB and marine bacterioplankton (in black). (b) Highlights the greater coverage (upper panel) and higher nucleotide identities (lower panel) of recruited reads against the Red Sea SAG both in the overlying 1500-m depth water column of Atlantis II Deep (A2D) and the next three transition layers (bsi, brine–seawater interface; ulc and lcl, upper and lower convective layers) of this brine pool. The blue dotted line demarcates the operational species-based nucleotide identity level for denoting similar genotypes as the reference. DD, Discovery Deep; KD, Kebrit Deep.