Figure 2.

Effects of papain pretreatment of isolated islets on prevention of lymphocyte proliferation against islet alloantigens Prior to the lymphocyte proliferation assay in vitro, BALB/c mouse islets were transplanted into C57BL/6J recipient mice to induce immunological sensitization. Ten days after transplantation, a lymphocyte proliferation assay was used to examine the proliferative response of the recipient spleen-derived lymphocytes to donor single islet cells (BALB/c). Proliferation of lymphocyte was measured by MTT assays after 24, 48, 72, 96, and 120 h of culture. The result of each well is presented as the mean ± SD (n = 7). The experiment was repeated 3 times. *p < 0 .001 compared with the corresponding 0 papain mg/mL group.