Figure 5.

Effect of lyngbic acid on QS pathways. Luminescence assays were performed in seven mutant strains and one wild-type strain of Vibrio with concentrations up to 2 μm lyngbic acid to determine its effect on several different pathways in the Vibrio model. luxM, cqsA and luxS encode synthases of the AHL, furanosyl borate diester AI-2 and hydroxyketone CAI-1 signals; luxN, cqsS and luxPQ encode receptors of these signals. LuxU is a phosphorelay protein through which inputs of all three QS systems are integrated into downstream regulatory cascades. Assays were performed in duplicates in three independent experiments. Heatmaps were constructed using data from a representative experiment after 8–10 h incubation with lyngbic acid.