Table 1. Growth of Dhc pure cultures amended with different cobamides.

Dhc strain (Electron acceptor)	Cobamidea	DMBb	Dhc cell density (16S rRNA gene copies per ml)		Growth yield (cells (μmol Cl-)-1)c
			Initial	Final
BAV1	DMB-Cba	−	4.26±0.87 × 106	1.82±0.25 × 108	1.38±0.09 × 108
(cDCE)	5-MeBza-Cba	−	4.26±0.87 × 106	2.09±0.51 × 108	1.54±0.39 × 108
	5-OMeBza-Cba	−	4.26±0.87 × 106	1.85±0.39 × 108	1.42±0.20 × 108
	Bza-Cba	−	4.26±0.87 × 106	2.07±0.10 × 108	1.57±0.03 × 108
GT	DMB-Cba	−	5.22±0.54 × 106	1.29±0.22 × 108	0.92±0.15 × 108
(cDCE)	5-MeBza-Cba	−	5.22±0.54 × 106	1.30±0.41 × 108	1.10±0.24 × 108
	5-OMeBza-Cba	−	5.22±0.54 × 106	4.97±0.99 × 107	6.95±1.04 × 107
	Bza-Cba	−	5.22±0.54 × 106	5.72±0.38 × 107	7.72±0.87 × 107
GT	DMB-Cba	−	2.61±0.27 × 106	0.96±0.06 × 108	1.17±0.15 × 108
(VC)	5-MeBza-Cba	−	2.61±0.27 × 106	1.10±0.10 × 108	1.39±0.20 × 108
	5-OMeBza-Cba	−	2.61±0.27 × 106	2.08±0.01 × 106	No growth
	5-OMeBza-Cba	+	2.61±0.27 × 106	1.21±0.34 × 108	1.45±0.33 × 108
	Bza-Cba	−	2.61±0.27 × 106	2.37±0.00 × 106	No growth
	Bza-Cba	+	2.61±0.27 × 106	1.22±0.08 × 108	1.48±0.11 × 108

Abbreviations: Bza, benzimidazole; Cba, cobamide; cDCE, cis-1,2-dichloroethene; Dhc, Dehalococcoides mccartyi; DMB, 5,6-dimethylbenzimidazole; 5-MeBza, 5-methylbenzimidazole; 5-OHBza, 5-hydroxybenzimidazole; 5-OMeBza, 5-methoxybenzimidazole; VC, vinyl chloride.

^aCobamide concentration was 36.9 nM.

^bDMB was supplied at 10 μM.

^cGrowth yield was estimated from the final cell density after complete or stalled cDCE or VC dechlorination.