Figure 5.

DnaA dependence is variable among cyanobacteria. (a) Phylogenetic tree of the phylum Cyanobacteria (black bar) and plastids (green bar) based on 16S rRNA alignment. (b) Cell growth in dnaA and POR disruptants of Synechocystis (upper) and a dnaA disruptant of Anabaena (lower). Data represent the mean of three biological replicates; error bars indicate s.d. (c) DNA replication activity as determined by western blot analysis of BrdU-labeled DNA in WT cells and Synechocystis (upper) and Anabaena (lower) deletion mutants. Signal intensity was quantified as arbitrary units (AU). The x axis shows periods of BrdU pulse labeling.