Fig. 1.

Differentiation of neural progenitor cultures. (A) NSCs from striatal tissues of P1 C57/B6 litters were differentiated and subsequently stained with antibodies reactive to either mouse NG2 or O1. (B) After 6 days, both NG2 and O1 cells were detected by immunofluorescent staining. In (A) and (B), nuclei were stained with DAPI (blue). (C) Frequency of NG2⁺O1⁻ and NG2⁻O1⁺ cells at days 0 and 6 postdifferentiation. Data represent the percentage of cells staining positive for either antigen and presented as average ± SD from two independent experiments. **Statistical significance for P values ≤0.001. For (A) and (B), scale bar = 40 μm.