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. 2016 Sep 20;6:33856. doi: 10.1038/srep33856

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) The Loxl2 mRNA level was significantly reduced in the Loxl3^−/− lungs compared with the Loxl3^+/+ lungs. *P < 0.05. Conversely, the Loxl4 mRNA levels in the Loxl3^−/− lungs were significantly increased compared with the Loxl3^+/+ lungs. **P < 0.01. n = 3 in each group. (B) The LOXL4 protein levels in the lung were analysed by Western blot and normalized to β-actin expression. Loxl3^−/− lungs contained higher levels of the LOXL4 protein than Loxl3^+/+ lungs. *P < 0.05, **P < 0.01. n = 5 in each group. (C–K) The distribution of LOXL4 in the Loxl3^+/+ and Loxl3^−/− lungs was analysed by immunofluorescence. (C–E) LOXL4 was found to express widely in the Loxl3^+/+ lung. (F–H) LOXL4 expression was not detected in the Loxl3^+/+ lung in the presence of the LOXL4 competing peptide. (I–K) The high expression of LOXL4 was also observed in the Loxl3^−/− lungs. DAPI was used to stain the nucleus. Bar: 100 μm.