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. 2016 May 18;7(7):826–835. doi: 10.1080/21505594.2016.1186324

Figure 1.

Figure 1.

The transcription factor Batf3 is critical for the accumulation of CD103+ DCs in the mouse kidney but dispensable for protection against systemic candidiasis. (A-D) Kidney leukocytes were isolated from Batf3+/+ and Batf3−/− mice left uninfected or infected intravenously with C. albicans 4 d prior to harvest, and analyzed using flow cytometry. (A-B) Representative zebra FACS plots from uninfected mice (A) or Candida-infected mice at day 4 p.i. (B) after gating on live CD45+MHCIIhighF4/80−CD11c+ DCs, and plotting based on CD103 and CD11b expression. (C) Total number of CD103+CD11b− DCs in the uninfected and Candida-infected kidney. (D) Percentage of CD103+CD11b− cells within all DCs in the uninfected and Candida-infected kidney. (E) Survival of Candida-infected Batf3+/+ and Batf3−/− mice. (F) Kidney fungal burden in Candida-infected Batf3+/+ and Batf3−/− mice determined at day 4 p.i. as the number of CFUs/gram of tissue. (G) Serum creatinine levels of Candida-infected Batf3+/+ and Batf3−/− mice at day 4 p.i. (H) Representative H&E stained kidney sections from Candida-infected Batf3+/+ and Batf3−/− mice at day 4 p.i. Data in (C, D, F, and G) were analyzed using unpaired t-tests or Mann-Whitney U tests, where appropriate. Data in (E) were analyzed using a log-rank (Mantel-Cox) test. Data in (A-D) are representative FACS plots or combined data from 2 independent experiments with a total n of 5–6 mice/group. Data in (E) are combined from 2 independent experiments with a total n of 19–21 mice/group. Data in (F-G) are combined from 2–3 independent experiments with a total n of 8–11 mice/group. Histology sections in (H) are representative images from 2 independent experiments with a total of 4–5 mice/group.