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. 2016 Sep 8;1(14):e88856. doi: 10.1172/jci.insight.88856

Figure 4. C19MC miRNA and GLUT-1 mRNA levels are significantly lower in propranolol-treated or partially involuted IH tumors.

Figure 4

Tissue from infants and children with proliferative IH (n = 5, green bars, mean age 5.34 mo), infants and children with proliferative IH who are undergoing propranolol therapy (n = 5, gray bars, mean age 16.0 mo), or infants and children with partially involuted IH (n = 6, black bars, mean age 58.9 mo) or adjacent skin (n = 5, pink bars) was analyzed using qRT-PCR for (A) 5 C19MC miRNAs (miR-517a-3p, miR-517c-3p, miR-518b, miR-519a-3p, and miR-520c-3p), (B) 4 non-C19MC miRNAs (miR-126-3p, miR-145-5p, miR-30b-5p, and miR-423-3p), and (C) GLUT-1 mRNA. Both the propranolol-treated and involuted tumors contained significantly lower levels of C19MC miRNAs, which were undetectable in skin, while non-C19MC miRNAs were detected in all tissues and levels were unaffected by involution or propranolol treatment. ANOVA analysis was used for comparison of all groups, and when deemed significant (P < 0.05) post-hoc Tukey’s test was performed to determine the significance of each group comparison (*P < 0.05; **P < 0.01). The y axis represents relative mRNA or miRNA compared with levels of GAPDH or the global mean of all miRNAs tested, respectively. Indicated data points represent individual patient samples, and error bars represent standard deviations.