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. Author manuscript; available in PMC: 2017 May 17.
Published in final edited form as: J Proteomics. 2016 Mar 26;140:24–36. doi: 10.1016/j.jprot.2016.03.032

Figure 6.

Figure 6

Western-blotting analysis of representative proteins identified by IMS-enhanced MSE.

Left panel: Western blotting analysis of CaMKII (A) and MBP (B) expression levels in the hippocampal tissues. Hippocampal protein lysate was loaded onto a SDS-PAGE gel and probed with antibody against CaMKII and MBP. β-actin was used as loading control and for data normalization; Right panel: bar chart of relative band intensity ratio of CaMKII versus reference protein (β-actin) and MBP versus reference. Average and standard deviation calculated from biological replicates (n=2 for CaMKII, n=3 for MBP).