FIG. 4.

Loss of pachytene spermatocytes and spermatids in the Mtor^flox/flox;Amhr2^cre/+ model. A and B) Photomicrographs show increased numbers of degenerating spermatocytes (arrows) in stage IX tubules of 3-mo-old Mtor^flox/flox;Amhr2^cre/+ mice relative to those of age-matched Mtor^flox/flox controls. Hematoxylin-eosin-phloxine-saffron stain. C) RT-qPCR analysis is shown of the indicated spermatogenesis marker genes in testes from 3-mo-old Mtor^flox/flox;Amhr2^cre/+ and age-matched Mtor^flox/flox mice (n = 5 animals/genotype). All data were normalized to the housekeeping gene Rpl19 and are expressed as mean (columns) ± SEM (error bars). *Significant differences from control (*P < 0.05; **P < 0.01; ****P < 0.0001).