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. 2016 Jun 8;95(1):13. doi: 10.1095/biolreprod.115.138016

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© 2016 by the Society for the Study of Reproduction, Inc.

This article is available under a Creative Commons License 4.0 (Attribution-Non-Commercial), as described at http://creativecommons.org/licenses/by-nc/4.0

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FIG. 6 — Mislocalization of GJA1 in testes of Mtor^flox/flox;Amhr2^cre/+ mice. A and B) Immunohistochemical analysis of GJA1 in testes from 3-mo-old Mtor^flox/flox;Amhr2^cre/+ and Mtor^flox/flox control mice. Scale bar in B is shown for both images. C) Immunoprecipitation of GJA1 followed by immunoblot analyses against GJA1 and the 14-3-3 mode 1 binding motif. Samples were total testicular protein extracts from 3-mo-old mice of the indicated genotypes (1 lane = 1 animal). Control immunoprecipitation reactions using IgG instead of the GJA1-specific antibody failed to immunoprecipitate 14-3-3 mode 1 binding motif-immunoreactive proteins (not shown). D) Immunoblot analysis of AKT and p (S473)-AKT in testes of 3-mo-old mice of the indicated genotypes is shown (1 lane = 1 animal). ACTB was used as the housekeeping control.