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. 2016 Oct;22(10):1550–1559. doi: 10.1261/rna.057679.116

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© 2016 Niinuma et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 3. — Deadenylation of the internalized poly(A) sequence involves the CCR4–NOT complex. (A) Western blot analysis. CCR4 and CAF1 were significantly reduced by treatment of the corresponding dsRNA to S2 cells. (B) Deadenylation assay for Mini-let-7-A₂₀ and Mini-let-7-A₂₀N₁₀ with S2 cell lysate knocked down CAF1. While mock knockdown lysate effectively induced deadenylation for both reporter RNAs, CAF1 knockdown lysate failed to deadenylate. (C,D) Deadenylation assay for Mini-let-7-A₂₀ and Mini-let-7-A₂₀N₁₀ with S2 cell lysate knocked down CCR4. Compared to mock knockdown, deadenylation was retarded in CCR4 knockdown.