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. 2016 Oct;22(10):1550–1559. doi: 10.1261/rna.057679.116

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© 2016 Niinuma et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 5. — The balance between the poly(A) length and the downstream post-poly(A) length affects the activity of CCR4 and CAF1. (A) Deadenylation of noC reporter RNAs with four types of recombinant CCR4/CAF1 heterodimer after 2 h incubation. (B) Deadenylation of noC-A₆₀C₁₀ and noC-CG₄₀A₂₀C₁₀ with four types of recombinant CCR4/CAF1 heterodimer after 2 h incubation. (C) The signal intensity of the bands in A was quantified, and the ratio of the intensity from the lower band for the total intensity was calculated and shown. As well as Figure 2C, noC-A₂₀ effectively shifted to A₀. While the length of the post-poly(A) sequence negatively correlated with the deadenylation efficiency, elongating the internal poly(A) sequence improved. The graph shows means and standard deviations (n = 3). (D) The signal intensity of the bands in B was quantified, and the ratio of the intensity from the lower band for the total intensity was calculated and shown. noC-A₆₀C₁₀ was deadenylated more effectively than noC-CG₄₀A₂₀C₁₀. The graph shows means and standard deviations (n = 3).