Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Oct;22(10):1592–1603. doi: 10.1261/rna.056895.116

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Baran-Gale et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

FIGURE 5. — Potential miR-503 targets. (A) The expression profile of 28 miR-503 targets mRNAs that are characteristic of the difference in gene expression between consecutive time points. The expression profiles of miRNAs were clustered using a hierarchical clustering method. (B) Expression profile of miR-503 and validated target, CCND1. (C) Expression profile of miR-503 and predicted target, ZNF217. (D) Expression profile of miR-503 and predicted target, RET. All plots (B–D) show the mean of three biological replicates as a blue line with box and whisker plots showing the variation in log₂ (fold change) between replicates. (E) miR-503 target site in the ZNF217 3′-UTR. A dual-luciferase reporter was used to validate the response of ZNF217 to miR-503. The reporter was mutated by inserting two A's (red) to disrupt the seed region binding of miR-503. (F) Response of the ZNF217 reporter and mutant reporter with and without 10 nM miR-503 mimic. Significance assessed using a Student's t-test.