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Four sites in T4 lysozyme (PDB‐ID 3fa0 [13]) mapped to four aliases in the script file wtT4L.py (see Supporting Information S2_ File). A: Site11, Glu11 in the active site.25 B: Site31, the His31‐Asp70 salt bridge that adds 3 − 5 kcal/mol of stability to T4 lysozyme.26 C: Site96, the site of the Arg96 →His mutant that suffers a large loss in stability.27 D: Site99, the site of a large cavity upon replacement of Leu99 with alanine.28
