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. 2016 Aug 13;25(10):1863–1872. doi: 10.1002/pro.2993

Figure 2.

Figure 2

Validation of the expression of membrane protein in S. cerevisiae in the micro‐plate format. (A) Illustration of the expression vector of the adenosine A2A receptor, pDDGFP2_A2AR. Yeast α‐factor secretion signal sequence (α‐factor) was needed for higher expression of A2AR. (B) FSEC chromatogram of A2AR‐WT. The insoluble materials were separated by the 0.22 μm filter (solid line) or by ultracentrifugation (dashed line). (C–F) The FSEC chromatogram of A2AR‐WT; (C), A2AR‐StaR2; (D), corticotropin‐releasing factor 1 receptor (CRF1R); (E), S. cerevisiae urea transporter (Dur3); (F) solubilized with (solid line) and without (dashed line) cell debris. The chromatograms detected by the UV–Vis detector at 280 nm are shown in the inset. Asterisks indicate the void peak. Arrows indicate the peak of the monodisperse membrane proteins.