Figure 3.

Cell growth of the preculture and expression level. (A) Cell growth of FGY217 yeast harboring the A_2A‐WT plasmid in −Ura + Ade medium with 2% glucose. The means and errors for the optical densities (ODs) at 600 nm from eight randomly selected wells were plotted at the indicated time after inoculation. (B) The cell density (optical density) of 24 colonies of A_2A‐WT and A_2A‐StaR2 after 9 h of first inoculation. The culture wells were divided into two groups: a high‐cell density group (OD₆₀₀ = 4.0–6.4, average 5.2) and a low‐cell density group (OD₆₀₀ = 0.6–2.9, average 2.2). (C) The peak heights of FSEC chromatograms of A_2A‐WT and A_2A‐StaR2 for the group of high cell density (white bar) and low cell density (black bar). Mean values ± the standard deviations (SD) are calculated from the eight wells of each group. The ODs of the culture in all wells were similar to each other at harvest.