Figure 5.

Assessment of the ligand binding activity of the receptor by FSEC. (A) (left panel) FSEC chromatogram of A_2A‐WT and the L48A mutant. (right panel) FSEC chromatogram of the L48A mutant with or without 10 μM ZM241385 during induction. (B) (left panel) FSEC chromatogram of A_2A‐WT and the A54L mutant. (right panel) FSEC chromatogram of the A54L mutant with or without 10 μM ZM241385 during induction. Arrows indicate the peak of A_2AR‐GFP. The mean values of the peak values derived from four colonies are indicated by horizontal lines. Each sample was centrifuged at 50,000 rpm for 30 min to eliminate insoluble materials before FSEC analysis.