Figure 3.

Human bone marrow–derived mesenchymal stem cells (hBMSCs) from donors #1, 21 years old; #2, 27 years old; #3, 31 years old at passage (P1) were used to assess population doubling (PD) per day described in Figure 1 , pellets glycosaminoglycan (GAG)/DNA (μg/μg), and donor #2 was histological safranin-O staining. (A) The proliferation rates of cells in condition 3, 5% fetal bovine serum (FBS) + 10 ng/mL fibroblast growth factor 2 (FGF-2) were higher than those in condition 1 5% FBS (*P < 0.05, t test). (B) No difference in GAG/DNA ratio was found between conditions 1 and 2, 10% FBS; the ratio in condition 3 was higher than the others (*P < .05, n = 3, assayed in triplicate, t test). (C) The safranin-O staining was more intense in condition 3 than in conditions 1 and 2.