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. 2016 Mar 30;7(17):23006–23018. doi: 10.18632/oncotarget.8470

Figure 1. Nbn depletion leads to premature hair loss due to depletion of Krt15+ and Cd34+ HF progenitors.

Figure 1

A. Hair loss in NbnEgr2-Cre skins from P35 to P90. Representative NbnCtrl and NbnEgr2-Cre mice at different age, P18, P35 and P90. The hair loss can be observed from P35. B. Histological analysis of NbnEgr2-Cre skin reveals progressive thickening of the epidermis and absence of HFs by P90. Scale bar 100 μm. C. Progressive depletion of Krt15+ HF progenitors and increase number of Krt14+ keratinocytes in NbnEgr2-Cre skins. Scale bar 20 μm. D. Sox9 immunostaining in NbnCtrl and NbnEgr2-Cre skins. E. Quantification of Krt15+, Sox9+ and Cd34+ cells in P18, P35 and P90 NbnEgr2-Cre skins. The Krt15+, Sox9+ and Cd34+ cells were count per skin length (mm). For Krt15: NbnCtrl P18 (N = 4), NbnEgr2-Cre P18 (N = 4), NbnCtrl P35 (N = 4), NbnEgr2-Cre P35 (N = 4), NbnCtrl P90 (N = 2), NbnEgr2-Cre P90 (N = 4). ***: p = 0.0003, **: p = 0.0018. For Sox9: NbnCtrl P18 (N = 2), NbnEgr2-Cre P18 (N = 3), NbnCtrl P35 (N = 3), NbnEgr2-Cre P35 (N = 3), NbnCtrl P90 (N = 3), NbnEgr2-Cre P90 (N = 3). **: p = 0.0021. For Cd34: NbnCtrl P18 (N = 2), NbnEgr2-Cre P18 (N = 3), NbnCtrl P35 (N = 3), NbnEgr2-Cre P35 (N = 2), NbnCtrl P90 (N = 2), NbnEgr2-Cre P90 (N = 4).*: p < 0.05.