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. 2016 Mar 30;7(17):23006–23018. doi: 10.18632/oncotarget.8470

Figure 2. Nbn deficiency in HF progenitors triggers DNA damage response and secretion of pro-inflammatory cytokines.

Figure 2

A. Increase of γ-H2afx foci and Trp53 stabilization in NbnEgr2-Cre HFs at P18. Scale bar 20 μm. B. Quantification of cleaved caspase 3 (Casp3+) in hair sections. NbnCtrl P18 (N = 3), NbnEgr2-Cre P18 (N = 4). C. Real-time RT-PCR analysis of Bax and Bbc3 (Puma) expression in NbnEgr2-Cre P18 skins. NbnCtrl P18 (N = 4), NbnEgr2-Cre P18 (N = 4). ***: p < 0.0001. D. Reduction of BrdU incorporation in NbnEgr2-Cre HF sections. NbnCtrl P18 (N = 3), NbnEgr2-Cre P18 (N = 4). *: p = 0.0108. E. Reduction of Ki67 positive (Ki67+) cells in NbnEgr2-Cre HF sections. NbnCtrl P18 (N = 3), NbnEgr2-Cre P18 (N = 4). *: p = 0.0108. Real-time RT-PCR analysis of Cdkn2a (p16INK4A) F. and Cdkn1a (p21Cip1/Waf1) G. expressions in NbnEgr2-Cre P18 and P35 skins. NbnCtrl (N = 4), NbnEgr2-Cre (N = 4). ***: p < 0.0001, **: p = 0.0077. Real-time RT-PCR analysis of pro-inflammatory cytokines: Ccl2 H., NbnCtrl P18 (N = 2), NbnEgr2-Cre P18 (N = 3), NbnCtrl P35 (N = 6), NbnEgr2-Cre P35 (N = 6); Il6 I. NbnCtrl P18 (N = 2), NbnEgr2-Cre P18 (N = 2), NbnCtrl P35 (N = 5), NbnEgr2-Cre P35 (N = 5); Tnf J., NbnCtrl P18 (N = 2), NbnEgr2-Cre P18 (N = 3), NbnCtrl P35 (N = 5), NbnEgr2-Cre P35 (N = 5) and Il1b K., NbnCtrl P18 (N = 2), NbnEgr2-Cre P18 (N = 3), NbnCtrl P35 (N = 6), NbnEgr2-Cre P35 (N = 6). ***: p < 0.0001, **: p = 0.0085, *: p < 0.05.