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. 2016 Mar 31;7(17):23056–23071. doi: 10.18632/oncotarget.8521

Figure 6. BMAL1 and RORα inhibition results in decreased expression of select HIF-1 target genes.

Figure 6

A.-B. qRT-PCR analysis of mRNA expression of select HIF-1 target genes following stable silencing of BMAL1 (A) or pharmacological inhibition of RORα activity by ML176 (10 μM) treatment. Knockdown of BMAL1 resulted in significant decrease in VEGF-A, enolase 1 (Eno1), PFKFB3 as well as RORα under both normoxia and hypoxia. B. NP cells treated with RORα inhibitor showed decreased mRNA expression of BMAL1, VEGF, PFKFB3 and Eno1. Expression of PHD2, another known HIF-1 target gene, remained unaffected in BMAL1 silenced and ML176 treated NP cells. Data is represented as mean ± SE. n=5, *p<0.1.