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. 2016 Mar 15;7(17):23239–23250. doi: 10.18632/oncotarget.8096

Figure 2. Emetine synergized with ara-C cytotoxicity in AML cells.

Figure 2

A. KG-1 cells were treated with 75 nM Emetine (E, red), 30 nM ara-C (A, blue) or a combination of both (E+A, dark grey). Each point represents a triplicate and error bars represent SEM. Y-axis: relative number of live cells refers to vehicle-treated samples assessed by flow cytometry (7-AAD−, Hoechstlow). Dotted lines represent the expected effect if drugs were additive (as predicted by Bliss additivity). B. HL-60 and KG-1 cells were treated with emetine in combination with ara-C at different concentrations for 24 h and cell viability was analyzed by flow cytometry. Tables show color-coded Excess Over Bliss Additivity (EOBA) for each combination assayed expressed as a percentage. Results are a mean of a triplicate. C. HL-60 and ara-C-resistant HL-60 (Ara-C-R) AML cell lines were treated for 24 h with increasing concentrations of emetine. Each point represents a triplicate and error bars represent SEM. Y-axis: number of live cells relative to untreated control as assessed by flow cytometry (7-AAD−, Hoechstlow)*p < 0.05; ** p < 0.005; ***p < 0.0005.