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. 2016 Mar 21;7(17):23395–23415. doi: 10.18632/oncotarget.8246

Figure 7. Radiation-induced NF-κB directly regulates TNFα transcription that acts in concert with BV6 to induce tumor cell death.

Figure 7

A. The human TNFα promoter structure. The three NF-κB-binding consensus sequence elements are indicated at the bottom of the bar. B. Tumor cells were irradiated at a dose of 50 Gy and then cultured for 90 min. Nuclear extracts were prepared and analyzed for NF-κB activation using EMSA with the three DNA probes of the human TNFα promoter as indicated in A. C. Tumor cells were irradiated with the indicated dose and cultured for 24h. Cells were then analyzed for human TNFα mRNA level using quantitative PCR. D. Tumor cells were treated as indicated for 24h and analyzed for cell death using PI staining and flow cytometry analysis. E. Tumor cells were irradiated and then cultured in the presence of BV6 (5 μM). IgG or anti-TNFα neutralization antibody (200 μg/ml) was added to the culture, respectively, for 24h. Cell death was analyzed by PI staining and flow cytometry analysis. *p < 0.05.