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. 2016 Mar 21;7(17):23395–23415. doi: 10.18632/oncotarget.8246

Figure 8. Radiation induces NF-κB to regulate FAS transcription.

Figure 8

A. The human FAS promoter structure. The NF-κB-binding consensus sequence element is indicated at the bottom of the bar. Tumor cells were irradiated at a dose of 50 Gy and then cultured for 90 min. Nuclear extracts were prepared and analyzed for NF-κB activation using EMSA with the DNA probe of the human FAS promoter as indicated in the top panel. B. HT1080 tumor cells were irradiated with the indicated doses and cultured for 24 hours. Cells were then analyzed for human Fas mRNA levels using quantitative PCR. C. HT1080 tumor cells were irradiated with the indicated doses for 24 hours. The cell surface Fas was stained with Fas-specific antibody and analyzed by flow cytometry. Representative plots are presented at the left. Fas MFI was quantified and presented at the right.