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. 2016 Mar 21;7(17):23395–23415. doi: 10.18632/oncotarget.8246

Figure 9. Radiation and TNFα induce apoptosis and necroptosis.

Figure 9

A. Tumor cells were irradiated (R) at a dose of 50 Gy and then cultured in the presence of BV6 (B, 5 μM) plus Z-VAD (Z, 10 μM), Nec-1 (10 μM), and NSA (2 μM), either alone or in combinations, for 24 hours. Cell death was determined by PI staining and flow cytometry analysis. Column: mean, bar: SD. B. Tumor cells were cultured in the presence of TNFα (T, 100 U/ml) and BV6 (B, 5 μM) plus Z-VAD (Z, 10 μM), Nec-1 (10 μM), and NSA (2 μM), either alone or in combinations, for 24h, and analyzed for cell death as in A. Column: mean; Bar: SD.