Table 1. Establishment of an in vivo metastasis model of human HCC cell line in nude mice.
Cell line | Injection site | Cell number | Number of nude mice injected (n) | Weeks | Hematogenic metastases | Subcutaneous tumor formation | ||||
---|---|---|---|---|---|---|---|---|---|---|
Lung | Liver | Spleen | Kidney | Heart | ||||||
Huh7CD133+ | i.v. | 106 | 10 | 10 | 7/10 | 0/10 | 0/10 | 0/10 | 0/10 | 2/10 |
Huh7CD133− | i.v. | 106 | 10 | 10 | 0/10 | 0/10 | 0/10 | 0/10 | 0/10 | 0/10 |
*i.v.: Intravenous injection.