Figure 2. Flow cytometric analysis of surface marker expression on breast cancer cell lines exposed to enzalutamide.

ZR75-1, BT549 and MDA MB 231 cells were exposed to 10 μM enzalutamide for 48 hours then analyzed by flow cytometry for cell surface expression of HLA A2, CEA, MUC-1, ICAM-1, Fas, and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptors 1 and 2. A. Percent positivity and relative surface expression on a per-cell basis (mean fluorescence intensity (MFI)), in parentheses, are shown. Values in bold denote an increase of > 15% relative to vehicle-treated cells. Data shown is representative of experiment repeated 3 times with similar results. B. Representative histograms of ICAM-1 and Trail R2 expression for MDA MB 231. *p < 0.05.