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. 2016 Mar 14;7(17):23512–23520. doi: 10.18632/oncotarget.8049

Figure 3. LT-NNK exposure enriched CSC properties with presentation of CSC-representative markers and sphere formation.

Figure 3

A. Overexpression of CSC-representative markers including Nanog and OCT4 was found in NNK-treated cells in a dose dependent manner, compared with control cells by Western blotting. B. LT-NNK exposure also demonstrates increased expression of CSC-representative markers including CD133, CD44 and CD24 by flow cytometry. C. Using a nonadhesive culture system, LT-NNK exposure is prone to form sphere, compared with the control cells.