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. 2016 Mar 14;7(17):23552–23568. doi: 10.18632/oncotarget.8083

Figure 1. KLF8 upregulates CXCR4 expression associated with invasive potential.

Figure 1

(A) Overexpression and knockdown of KLF8 induces and reduces CXCR4 expression in the 10A-iK8 and 231-K8ikd cells, respectively. Total RNA and protein lyaste were prepared from sub-confluent cells grown under uninduced (U) and doxycycline-induced (I) conditions. The levels of CXCR4 were determined by qRT–PCR (top graph), semi-quantitative RT–PCR (middle panel) or western blotting (bottom panel). Overexpression of HA-KLF8 and knockdown of KLF8 were examined by western blot. (B) Aberrant co-elevation of KLF8 and CXCR4 protein in the patient invasive breast carcinoma (IBC) tumors. IHC staining was performed for KLF8 or CXCR4 (brown) in the human breast cancer tissue microarray containing specimens in duplicate from 75 patient tumors or normal tissues. Images representing a benign sample negative for both KLF8 and CXCR4 (case 1) and an IBC sample positive for both KLF8 and CXCR4 (case 2) are shown. Correlation of CXCR4 and KLF8 expression is outlined in the tables. (C) Aberrant co-elevation of KLF8 and CXCR4 mRNA in the patient IBC tumors. Oncomine analysis was performed on an independent cohort of Finak dataset containing 6 normal and 53 IBC samples. Pearson's correlation of KLF8 and CXCR4 was applied to Finak dataset downloaded from GEO (GSE9014). (D) The expression of KLF8 and CXCR4 is correlated in invasive breast cancers. RNA sequencing data downloaded from TCGA breast cancer (2015) were analyzed.