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. 2016 Mar 14;7(17):23552–23568. doi: 10.18632/oncotarget.8083

Figure 7. KLF8 activation of CXCR4/CXCL12 signaling is required for lung metastasis.

Figure 7

(A, B) Overexpression of CXCR4 but not the dN20 mutant prevents lung metastasis from inhibition by KLF8 knockdown. The 231-K8ikd, 231-K8ikd/CXCR4 and 231-K8ikd/dN20 cells were injected into the tail vein of the mice. The mice were fed with Dox-diet (I) or control diet (U). The lung metastatic rate was followed up for 5 weeks by BLI analysis (a). *P < 0.05 between the U and I groups and between I + CXCR4 and I + dN20 groups. Representative BLI images, metastatic nodules on the whole mount lungs and statistic values in the end of week 5 are shown (B). (C) Histological evidence supporting the role of CXCR4 downstream of KLF8 for lung metastasis. The above-described lungs were processed for H/E and IHC staining with antibodies against GFP and human vimentin.