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. 2016 Mar 14;7(17):23668–23683. doi: 10.18632/oncotarget.8056

Figure 4. IKBKB plays a critical role in Taxol-induced apoptosis.

Figure 4

A. MDA-MB-231 and MCF-7 cells transfected with blank-plasmid or IKBKB (inset) overexpression plasmid were seeded into 96-well plates and treated with 0, 10, 20, 40, 80 nM (MDA-MB-231) or 0, 100, 200, 400, 800 nM (MCF-7) Taxol for 48 h. The inhibition of cell viabilities was detected using MTT assays. Columns, means of three independent experiments; bars, S.E. *p<0.05. B-C. MDA-MB-231 and MCF-7 cells were transfected with blank-plasmid or IKBKB overexpression plasmid and then treated with 40 and 400 nM Taxol for 48 h, respectively. Cell lysates were extracted for western blotting using antibodies against c-PARP and IKBKB (B), or cells were collected for annexin V staining and flow cytometry assays (C). The percentage of apoptotic cells is represented in a bar diagram from three independent experiments (C, right). β-actin was used as a loading control. Columns, means of three independent experiments; bars, S.E. *, p<0.05, **, p<0.01.