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. 2016 Mar 17;7(17):23684–23699. doi: 10.18632/oncotarget.8152

Figure 7. Effect of escin Ia on epithelial-mesenchymal transition in LOXL2-transfected MCF-7 cells.

Figure 7

MCF-7 cells were transfected with a plasmid of LOXL2 (P3.1-H (+) H_LOXL2) plus escin Ia (2.5, 5, 10 μM) or escin Ia (10 μM) for the indicated internals. (A) Cell morphology was recorded under an inverted microscope (magnification 200 ×). (B) Cell invasion was detected by using cell invasion assay. (C) Cell migration was detected by using cell migration assay. (D) The protein expressions of E-cadherin and vimentin were detected by using immunofluorescence assay. (E) The protein expressions of E-cadherin, vimentin and α-SMA were detected using western blot analysis. (F, H) The mRNA expressions of E-cadherin, Snail, Slug, Zeb1, Zeb2 and Twist were detected using Q-PCR assays. Gene expressions were normalized to GAPDH. (G) Time course of escin Ia on protein expressions of E-cadherin, vimentin and α-SMA were detected by using western blot analysis. The data were expressed as the means ± S.E.M. of three independent experiments. #p < 0.05, ##p < 0.01 vs. p3.1-H(+); *p < 0.05, **p < 0.01 vs. p3.1-H(+) H_LOXL2.