Figure 1. Acquired resistance mutations in patient-derived ALK+ ALCL cell lines.

(A) IC50s of parental ALK+ ALCL cell lines (SUP-M2 and SU-DHL-1) as well as an ALK-negative ALCL line (MAC-2A). Mean ± SEM for quadruplicates. (B) Sanger sequencing identifying each resistance mutation in cell lines. (C) Location of the five mutations identified in this study with respect to the ALK kinase domain shown as ball and stick models with associated surfaces colored by atoms.