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. 2016 Mar 14;7(17):23740–23756. doi: 10.18632/oncotarget.8060

Figure 3. Cancer cell-secreted VEGF modulated by DEK regulates HUVEC proliferation and migration.

Figure 3

A. Cell proliferation assays were performed in HUVEC cells cultured in conditioned medium from MCF-7 cells stably infected with lentivirus carrying DEK shRNA, DEK shRNA plus DEK-resistant shRNA (DEK-R), HIF-1α shRNA or DEK shRNA plus HIF-1α shRNA under hypoxic condition. Representative immunoblot shows the expression of DEK and HIF-1α. B. Cell proliferation assays were performed in HUVEC cells cultured in conditioned medium from MCF-7 cells stably transfected with DEK or empty vector under hypoxic condition and treated with a VEGF neutralizing antibody (α-VEGF). Representative immunoblot indicates the expression of DEK. *P < 0.05, **P < 0.01 (A, B). C. Wound healing assays were performed for HUVEC cells cultured in conditioned medium from MCF-7 cells stably infected as in A. D. Wound healing assays were performed for HUVEC cells cultured in conditioned medium from MCF-7 cells stably transfected and treated as in B. The image displayed is one of the representative results (C, D). Scale bar: 100 μm. All values shown are mean ± SD of triplicate measurements and have been repeated 3 times with similar results (C, D). *P < 0.05, **P < 0.01 versus empty vector or control shRNA plus empty vector.