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. 2016 Mar 16;7(17):23757–23771. doi: 10.18632/oncotarget.8121

Figure 3. Upregulation of E2F8 promotes proliferation of breast cancer cells.

Figure 3

(A) GSEA plot performed using GSEA v2.2.0 (http://www.broadinstitute.org/gsea/) showed that high E2F8 expression was positively correlated with the cell cycle-associated gene signature (BENPORATH_PROLIFERATION) based on TCGA BRCA mRNA data set. (B) Correlations between E2F8 and MKI67 mRNA expression based on TCGA BRCA mRNA data set. r, Pearson correlation coefficient; P < 0.001. (C) The protein expression levels of E2F8 were associated with the expression of Ki67 in 187 primary breast cancer specimens. Percentage of specimens showing low or high Ki67 expression (Ki67-L and Ki67-H) in 187 primary human breast cancer specimens, related to the levels of E2F8. P < 0.001, χ2 test. (D) Western blotting analysis of E2F8 protein expression in the constructed MCF7 and SK-BR-3 cells; α-tubulin was used as a loading control. (E) Proliferation rate of the indicated breast cancer cells, as determined using the MTT assay. (F) Representative images (left panel) and quantification (right panel) of crystal violet-stained colony formation for the indicated cell lines. Data are mean ± SD of three independent experiments. *P <0.05.