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. 2016 Mar 14;7(17):23837–23849. doi: 10.18632/oncotarget.8070

Figure 1. PIM1 association with the ribosome.

Figure 1

Cytoplasmic extracts from HCT116 and HEK293 transfected with HA-PIM1 were separated by ultra- centrifugation to a pellet (P), containing ribosomes and ribosomal subunits and a supernatant (S), containing free cytoplasmic proteins. The two fractions were analyzed by western blot with primary antibodies against PIM1, RPS19, β-Actin (ACT) and Neomycin phosphotransferase (NPT, encoded by the expression vector). The loading ratio between P and S was 3:1. In the left panel, PIM1 antibody detects endogenous protein whereas in the right panel the transfected HA-PIM1.