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. 2016 Mar 14;7(17):23837–23849. doi: 10.18632/oncotarget.8070

Figure 2. Analysis of PIM1 and p53 levels.

Figure 2

Total extracts were analyzed by western blot with indicated primary antibodies. Quantification of proteins from at least three independent experiments are reported in lower panels as a column plot of the mean±s.e.m. of the densitometry values normalized by GAPDH. a. Extracts from HCT116, LNCaP, 22Rv1, PC3 and MCF7 cells transfected with indicated siRNA (PIM1 analysis in LNCaP and 22Rv1 is the mean of two experiments). b. Extracts from HCT116 cells transfected with control (siCNT) or with PIM1-specific siRNA (siPIM). c. Extracts from HCT116 cells transfected first with RPS19 specific siRNA (siS19) and then transduced with PIM1-expressing lentivirus. Open triangles indicate non-specific bands; filled triangles indicate PIM1 bands.